Catalog Number - 68322-25P

68322-25P Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.

The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. It also serves as a suitable alternate column for USP methods where PFP selectivity is advantageous.

With a 3 µm particle size, this column offers higher efficiency than 5 µm columns of the same phase, delivering sharper peaks and improved resolution. As expected, the smaller particle size increases backpressure compared to larger particle columns.

250 mm Length – Maximum Resolution for the Most Demanding Separations

The 250 mm length provides the highest resolving power, making it ideal for complex mixtures and challenging separations.

• Maximum resolution compared to shorter column lengths

• Superior separation of closely related and co-eluting compounds

• Increased peak capacity for complex samples

✅ Recommended for impurity profiling, method development, and highly complex analyses

Flow Rate & System Requirements (4.6 mm ID, 3 µm)

⚠️ Compatible with standard analytical HPLC systems, with higher backpressure due to column length and particle size

• Typical flow rate range: ~0.8 – 1.5 mL/min

• Higher efficiency than 5 µm columns

• Increased backpressure from 3 µm particles and 250 mm length

• Suitable for UV and standard analytical detectors

✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) Columns for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with halogenated and fluorinated analytes

• Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

Versatile 100 Å Pore Structure

• Suitable for small to moderately sized molecules

• Balanced performance across applications

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry

High Efficiency (3 µm Particles)

• More efficient than 5 µm columns

• Sharper peaks and improved resolution

High Purity Silica

• Consistent and reproducible chromatographic performance

• Long column lifetime

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Polar compound retention

• Pharmaceutical QA/QC workflows

• Method development and impurity profiling

• USP alternate methods

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Higher backpressure due to particle size and column length

• Use 0.2 µm filtration prior to injection